pgl 2 basic luciferase (Addgene inc)
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Addgene inc
pgl 2 basic luciferase
Pgl 2 Basic Luciferase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl+2+basic+luciferase/pLL3%2E7+(Plasmid+%2311795)/pm32289262-260-130-125
Average 95 stars, based on 357 article reviews
Pgl 2 Basic Luciferase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl+2+basic+luciferase/pLL3%2E7+(Plasmid+%2311795)/pm32289262-260-130-125
Average 95 stars, based on 357 article reviews
pgl 2 basic luciferase - by Bioz Stars,
2026-09
95/100 stars
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Dominant Negative Mutation:Article Title: Neisseria gonorrhoeae Blocks Epithelial Exfoliation by Nitric-Oxide-Mediated Metabolic Cross Talk to Promote Colonization in Mice. Article Snippet: Meyer, MPI for Infection Biology, Berlin, Germany N280 Escherichia coli AfaE-III Muenzner et al., 2016 A30 Escherichia coli DAfaE-III Muenzner et al., 2016 A30-1 Escherichia coli BL21(DE3) Novagen, Madison, Wisconsin Cat # 69450 Inhibitors PKG inhibitor; 8-(4-chlorophenylthio)-guanosine 3’,5’- cyclic monophosphothioate Rp-isomer Calbiochem, Darmstadt, Germany Cat # 370655 PKA inhibitor; 14-22 Amide Calbiochem Cat # 476485 PKC inhibitor; chelerythrine chloride Calbiochem Cat # 220285 CaMKII iInhibitor; KN-62 Calbiochem Cat # 422706 sGC inhibitor; oxadiazolo [4, 3-a] quinoxalin-1one (ODQ) Calbiochem Cat # 495320 sGC agonist; BAY 41-2272 Calbiochem Cat # 196876 iNOS inhibitor; N,N-dimethyl-L-arginine (ADMA) Cayman Chemical (BIOMOL GmbH, Hamburg, Germany Item No. 80230 DETA Nonoate Cayman Chemical Item No. 82120 Recombinant Proteins, Chemicals recombinant AniA wt; AniA H280L this manuscript N/A 17-b-Estradiol Calbiochem Cat # 3301 Trimethoprim 98% Acros Organics, Geel, Belgium Cat # 455120050 IPTG Roth Cat # 206-703-0 (Continued on next page) Cell Host & Microbe 27, 1–16.e1–e5, May 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER GC-Agar Becton Dickinson Cat # 228950 OptiMEM Life Technologies, Darmstadt, Germany Cat # 31985062 DMEM Merck Cat # FG0435 Fetal calf serum Biochrom Cat # S0115 D-luciferin Thermo Fisher Scientific Cat # L2916 Griess reagent kit LifeTechnologies Ca t# G7921 Tissue-Tec Cryo-M-Bed; Bright Instrument, Huntingdon, UK No. 1321700001 Plasmids Constitutive active PKG Ia Deguchi et al., 2002 Addgene (Cambridge, MA) # 16392 Constitutive active PKG Ib Deguchi et al., 2002 Addgene (Cambridge, MA) # 16396 pRSV CREB S133A (dominant-negative CREB) Gonzalez and Montminy, 1989 Addgene (Cambridge, MA) # 22395 psPAX2 psPAX2 was a gift from Didier Trono Addgene (Cambridge, MA) # 12260 pMD2.G pMD2.G was a gift from Didier Trono Addgene (Cambridge, MA) # 12259 pLL3.7 Rubinson et al., 2003 Addgene (Cambridge, MA) # 11795 Luciferase:Article Title: Neisseria gonorrhoeae Blocks Epithelial Exfoliation by Nitric-Oxide-Mediated Metabolic Cross Talk to Promote Colonization in Mice. Article Snippet: Meyer, MPI for Infection Biology, Berlin, Germany N280 Escherichia coli AfaE-III Muenzner et al., 2016 A30 Escherichia coli DAfaE-III Muenzner et al., 2016 A30-1 Escherichia coli BL21(DE3) Novagen, Madison, Wisconsin Cat # 69450 Inhibitors PKG inhibitor; 8-(4-chlorophenylthio)-guanosine 3’,5’- cyclic monophosphothioate Rp-isomer Calbiochem, Darmstadt, Germany Cat # 370655 PKA inhibitor; 14-22 Amide Calbiochem Cat # 476485 PKC inhibitor; chelerythrine chloride Calbiochem Cat # 220285 CaMKII iInhibitor; KN-62 Calbiochem Cat # 422706 sGC inhibitor; oxadiazolo [4, 3-a] quinoxalin-1one (ODQ) Calbiochem Cat # 495320 sGC agonist; BAY 41-2272 Calbiochem Cat # 196876 iNOS inhibitor; N,N-dimethyl-L-arginine (ADMA) Cayman Chemical (BIOMOL GmbH, Hamburg, Germany Item No. 80230 DETA Nonoate Cayman Chemical Item No. 82120 Recombinant Proteins, Chemicals recombinant AniA wt; AniA H280L this manuscript N/A 17-b-Estradiol Calbiochem Cat # 3301 Trimethoprim 98% Acros Organics, Geel, Belgium Cat # 455120050 IPTG Roth Cat # 206-703-0 (Continued on next page) Cell Host & Microbe 27, 1–16.e1–e5, May 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER GC-Agar Becton Dickinson Cat # 228950 OptiMEM Life Technologies, Darmstadt, Germany Cat # 31985062 DMEM Merck Cat # FG0435 Fetal calf serum Biochrom Cat # S0115 D-luciferin Thermo Fisher Scientific Cat # L2916 Griess reagent kit LifeTechnologies Ca t# G7921 Tissue-Tec Cryo-M-Bed; Bright Instrument, Huntingdon, UK No. 1321700001 Plasmids Constitutive active PKG Ia Deguchi et al., 2002 Addgene (Cambridge, MA) # 16392 Constitutive active PKG Ib Deguchi et al., 2002 Addgene (Cambridge, MA) # 16396 pRSV CREB S133A (dominant-negative CREB) Gonzalez and Montminy, 1989 Addgene (Cambridge, MA) # 22395 psPAX2 psPAX2 was a gift from Didier Trono Addgene (Cambridge, MA) # 12260 pMD2.G pMD2.G was a gift from Didier Trono Addgene (Cambridge, MA) # 12259 pLL3.7 Rubinson et al., 2003 Addgene (Cambridge, MA) # 11795 Construct:Article Title: Neisseria gonorrhoeae Blocks Epithelial Exfoliation by Nitric-Oxide-Mediated Metabolic Cross Talk to Promote Colonization in Mice. Article Snippet: Meyer, MPI for Infection Biology, Berlin, Germany N280 Escherichia coli AfaE-III Muenzner et al., 2016 A30 Escherichia coli DAfaE-III Muenzner et al., 2016 A30-1 Escherichia coli BL21(DE3) Novagen, Madison, Wisconsin Cat # 69450 Inhibitors PKG inhibitor; 8-(4-chlorophenylthio)-guanosine 3’,5’- cyclic monophosphothioate Rp-isomer Calbiochem, Darmstadt, Germany Cat # 370655 PKA inhibitor; 14-22 Amide Calbiochem Cat # 476485 PKC inhibitor; chelerythrine chloride Calbiochem Cat # 220285 CaMKII iInhibitor; KN-62 Calbiochem Cat # 422706 sGC inhibitor; oxadiazolo [4, 3-a] quinoxalin-1one (ODQ) Calbiochem Cat # 495320 sGC agonist; BAY 41-2272 Calbiochem Cat # 196876 iNOS inhibitor; N,N-dimethyl-L-arginine (ADMA) Cayman Chemical (BIOMOL GmbH, Hamburg, Germany Item No. 80230 DETA Nonoate Cayman Chemical Item No. 82120 Recombinant Proteins, Chemicals recombinant AniA wt; AniA H280L this manuscript N/A 17-b-Estradiol Calbiochem Cat # 3301 Trimethoprim 98% Acros Organics, Geel, Belgium Cat # 455120050 IPTG Roth Cat # 206-703-0 (Continued on next page) Cell Host & Microbe 27, 1–16.e1–e5, May 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER GC-Agar Becton Dickinson Cat # 228950 OptiMEM Life Technologies, Darmstadt, Germany Cat # 31985062 DMEM Merck Cat # FG0435 Fetal calf serum Biochrom Cat # S0115 D-luciferin Thermo Fisher Scientific Cat # L2916 Griess reagent kit LifeTechnologies Ca t# G7921 Tissue-Tec Cryo-M-Bed; Bright Instrument, Huntingdon, UK No. 1321700001 Plasmids Constitutive active PKG Ia Deguchi et al., 2002 Addgene (Cambridge, MA) # 16392 Constitutive active PKG Ib Deguchi et al., 2002 Addgene (Cambridge, MA) # 16396 pRSV CREB S133A (dominant-negative CREB) Gonzalez and Montminy, 1989 Addgene (Cambridge, MA) # 22395 psPAX2 psPAX2 was a gift from Didier Trono Addgene (Cambridge, MA) # 12260 pMD2.G pMD2.G was a gift from Didier Trono Addgene (Cambridge, MA) # 12259 pLL3.7 Rubinson et al., 2003 Addgene (Cambridge, MA) # 11795 |